Microbial yield from infectious tissues pretreated by various methods: an invitro study

Abstract Background This study aimed to evaluate the effects of different pretreatment methods on microbial yield from infectious tissues. Methods Strains Staphylococcus aureus (SA), Escherichia coli (EC) and Candida albicans (CA) were used construct single-surface, full-surface, internal infection models in sterile pork tissue. Manual milling (MM), mechanical homogenization (MH), sonificated (SF), dithiothreitol (DTT), direct culture (DC) pretreat these tissues, recorded compared. Moreover, periprosthetic tissues collected intraoperatively joint (PJI) patients as a verification. Results The showed that MH was significantly higher than MM ( P < 0.01) SF method 0.01). Furthermore, model, group also 0.01), DTT DC number bacterial colonies obtained pretreated by other = 0.004). Conclusions DC, MM, can be process multiple tissue samples at same time, which might further improve diagnostic sensitivity disease.